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Figure S1 - Ionizing Radiation Induces Stemness in Cancer Cells

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posted on 2012-08-21, 00:14 authored by Laura Ghisolfi, Andrew C. Keates, Xingwang Hu, Dong-ki Lee, Chiang J. Li

HepG2 and Huh7 cell radio-sensitivity increases with gamma radiation dose. HepG2 cells and Huh7 cells were exposed to increasing doses of gamma radiation and then plated onto 96-well plates for viability evaluation by MTT assay (A and B). MTT assay was performed after 6 days of culture and an LD50 = 4.33 or LD50 = 4.48 Gy were observed for HepG2 and Huh7 cells, respectively. The viable fraction of cells at each radiation dose, expressed as natural log is plotted on the graphs. Lines were fitted using a first-order polynomial regression. Results are presented as the mean±SEM of three independent experiments where each radiation treatment group was seeded in triplicate.



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