Figure S1 - Ionizing Radiation Induces Stemness in Cancer Cells

HepG2 and Huh7 cell radio-sensitivity increases with gamma radiation dose. HepG2 cells and Huh7 cells were exposed to increasing doses of gamma radiation and then plated onto 96-well plates for viability evaluation by MTT assay (A and B). MTT assay was performed after 6 days of culture and an LD₅₀ = 4.33 or LD₅₀ = 4.48 Gy were observed for HepG2 and Huh7 cells, respectively. The viable fraction of cells at each radiation dose, expressed as natural log is plotted on the graphs. Lines were fitted using a first-order polynomial regression. Results are presented as the mean±SEM of three independent experiments where each radiation treatment group was seeded in triplicate.

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