Fig 5.tif (945.18 kB)

Investigation of autophagy.

Download (945.18 kB)
figure
posted on 07.03.2018, 18:35 by Lara Marrone, Christine Bus, David Schöndorf, Julia Catherine Fitzgerald, Manuela Kübler, Benjamin Schmid, Peter Reinhardt, Lydia Reinhardt, Michela Deleidi, Tanya Levin, Andrea Meixner, Barbara Klink, Michael Glatza, Christian Johannes Gloeckner, Thomas Gasser, Jared Sterneckert

(A) Representative western blot of LC3B-I, LC3B-II and GAPDH loading control in DANs derived from 4 iPD patients and 4 healthy controls. Labels C/C (risk allele) and T/T (non-risk allele) denote the genotype of iPD patients at LRRK2-RS1491923. mDANs were treated with a DMSO vehicle control or 100 nM bafilomycin (baf) for 24 h. (B) Western blots from three independent experiments (n = 3) were quantified by densitometry and LC3B-II normalized to a loading control. All measurements are shown. Data is grouped as healthy vs iPD as well as iPD stratified for T/T and C/C genotype. (C) Ratio of LC3B-II (normalized to loading control) following bafilomycin treatment/untreated. (D) Representative western blot of LAMP1 and α-tubulin loading control in mDANs derived from iPD patients and healthy controls. (E) Western blots from three independent experiments (n = 3) were quantified by densitometry and LAMP1 normalized to a loading control. All data is shown, including data stratified for T/T and C/C genotype. (F) Ratio of LAMP1 (normalized to loading control) following bafilomycin treatment/untreated.

History

Licence

Exports