Introducing N125D, D127E, D222G and R223Q to A/SLOV15 HA protein improved thermal stability.
figure
posted on 2022-05-27, 17:40 authored by Rachael Dempsey, Giulia Tamburrino, Katarzyna E. Schewe, Jonathan Crowe, Annalisa Nuccitelli, Oliver DibbenAccelerated thermostability assay was performed with A/SLOV15 V1 (blue), A/SLOV15 S13 (purple), A/New Caledonia/20/1999 (A/NC99, black) and A/California/07/2009 (A/CA09, red) LAIV viruses. A/SLOV15 V1, A/SLOV15 S13, A/NC99 and A/CA09 viruses were held at temperatures ranging from 47.5°C—65°C for 20 minutes. After incubation, all samples underwent haemagglutination assays using 0.5% chicken red blood cells and log2 titres were recorded.
History
Usage metrics
Categories
Keywords
without compromising yieldviruses replicated inefficiently>) reference strains2013 – 14sln ), relativeproduced approximately 1000optimise hnec replicationenhance hnec replicationsln binding networksln binding energyrational ha mutagenesisreduced vaccine effectivenessh1n1pdm09 vaccine straindiv >< p2015 – 16four ha substitutions6 receptor analogues13 protected ferretshaemagglutinin substitutions n125dvaccine effectivenesssln avidityr223q substitutionsbinding aviditystrain competitionhnec infectionhnec ).approximately 106 receptorwt type (<slov15 s13slov15 ).silico quadrivalent formulationsqlaiv ).possible mechanismovercome competitionmild increasemaintaining avianlike αlike 3later validatedimproved efficacyfever postdespite s13antigenically similarantigenic match
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC