Interaction of G-PUR® with sodium deoxycholate and its ability to bind C. difficile toxins.
A: An equimolar mixture of Clostridioides difficile toxins A and B was incubated with G-PUR® in the presence or absence of 0.1% (2.4 mM) and 0.01% (0.24 mM) deoxycholate or of bile salts. After incubation at 37°C, G-PUR® was pelleted by centrifugation and the concentration of clostridial toxins remaining in the supernatant was determined by ELISA. Data from 3 independent experiments carried out in triplicate are displayed as means ± standard deviations. B: [3H]deoxycholate was incubated at different concentrations in synthetic intestinal fluid containing 20 mg/ml G-PUR®. After a 2-hour incubation at room temperature, bound and unbound deoxycholate were separated by centrifugation and the free radioactivity was quantified by liquid scintillation. Data obtained from 3 independent experiments are shown as means ± standard deviations.