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Interaction of BTAF1 with mutant TBP proteins

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posted on 2011-12-30, 15:14 authored by Marcin P. Klejman, Xuemei Zhao, Frederik M. A. van Schaik, Winship Herr, H. Th. Marc Timmers

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Taken from "Mutational analysis of BTAF1–TBP interaction: BTAF1 can rescue DNA-binding defective TBP mutants"

Nucleic Acids Research 2005;33(17):5426-5436.

Published online 22 Sep 2005


© The Author 2005. Published by Oxford University Press. All rights reserved

() Representative results of BTAF1–TBP interaction assay in solution. Binding reactions were performed as described in Materials and Methods. Lane 5 contained wild-type TBP (50 ng), lanes 6–19 contained indicated mutant TBP proteins (50 ng) and no TBP was added to lane 20. Lanes 5–20 contained 5 µl of BTAF1-enriched lysate. BTAF1 and TBP proteins were co-immunoprecipitated using TBP antibody. Indicated amounts of BTAF1-containing lysate were loaded as the input control (lanes 1–4). Proteins were visualized using specific antibodies. () Human TBP model () showing results of BTAF1–TBP interaction. Top left—view from upstream DNA; top right—view from downstream DNA (start site); bottom left—view on the top (convex) TBP surface; and bottom right—view on the DNA-binding (concave) TBP surface. Residues affected in BTAF1–TBP interaction by mutations in TBP are indicated (, column 2): red,


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