Detection of nectin-1 and nectin-3 on K562 and NK-92 cells.
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posted on 2019-02-13, 18:52 authored by Veronica M. Holmes, Carlos Maluquer de Motes, Paige T. Richards, Jessenia Roldan, Arjun K. Bhargava, Jordan S. Orange, Claude KrummenacherWild type K562 cells (top) and NK-92 cells (bottom) were analyzed for expression of nectin-1 (left) and nectin-3 (right) by flow cytometry. Staining with anti-nectin-1 CK41 Mab and anti-nectin-3 N3.12.4 Mab were compared to isotype control staining with anti-FLAG M2 Mab. The control histograms are colored gray, the nectin-1 histograms are colored blue and the nectin-3 histograms are colored red. The anti-nectin-1 CK41, which yielded no staining of K562 and NK-92 cells was active and able to stain control C10 cells in this experiment (not shown).
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