pone.0207315.g004.tif (1.69 MB)

Interaction between MRE11, RAD50 and Xrs2/Nibrin.

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posted on 2018-11-15, 18:55 authored by Ilja Demuth, Simon K. Krebs, Véronique Dutrannoy, Christian Linke, Sylvia Krobitsch, Raymonda Varon, Christine Lang, Andreas Raab, Karl Sperling, Martin Digweed

HEK293 cells transiently expressing Flag-tagged nibrin or codon optimised synthetic Xrs2 were probed by co-immunoprecipitation using anti-Flag antibodies and western blot analysis for RAD50 and MRE11. As a control, aliquots of the lysates were analysed for MRE11 by immunoblotting.