posted on 2018-11-15, 18:55authored byIlja Demuth, Simon K. Krebs, Véronique Dutrannoy, Christian Linke, Sylvia Krobitsch, Raymonda Varon, Christine Lang, Andreas Raab, Karl Sperling, Martin Digweed
HEK293 cells transiently expressing Flag-tagged nibrin or codon optimised synthetic Xrs2 were probed by co-immunoprecipitation using anti-Flag antibodies and western blot analysis for RAD50 and MRE11. As a control, aliquots of the lysates were analysed for MRE11 by immunoblotting.