Interaction between Antibacterial Peptide Apep10 and Escherichia coli Membrane Lipids Evaluated Using Liposome as Pseudo-Stationary Phase - Fig 1

Electropherograms of Apep10 and acetone in the running buffer with various concentrations of (A) the E.coli membrane lipid liposome and (B) the mixed phospholipids liposome at 303 K. The liposome concentrations (mg/ml) were: Ⅰ, 0; Ⅱ, 0.1; Ⅲ, 0.2; Ⅳ, 0.4; Ⅴ, 0.8; Ⅵ, 1.2; Ⅶ, 1.6. The electrophoresis conditions used were as follows: injection, 0.8 psi for 6 s; separation voltage, 25 kV; detection, UV detection at 196 nm; and capillary, 65 cm × 75 μm inner diameter. Peaks (a) and (b) were Apep10 and acetone, respectively.