Integrated analysis of DEGs in WT and NOS1^−/− macrophages post-LPS stimulation.

A and B, Venn diagram showing the total number of genes upregulated (more than 2-fold) and downregulated (less than 0.5-fold) in WT and NOS1^−/− BMDMs treated with LPS for 2 (A) and 8 h (B) compared with untreated cells (0h). Each portion of a Venn diagram displays the number of DEGs in NOS1^−/− BMDMs compared to WT BMDMs. C and D, Histogram showing the number of genes upregulated (red) and downregulated (green) in NOS1^−/− compared with WT BMDMs post 2 and 8 h of LPS stimulation. E and F, Heat map of DEGs in macrophages with 2 and 8 h of LPS stimulation or without LPS stimulation. Each row represents a gene, and each column represents a time point.