Inhibition of IKZF1, IRF4, and cMyc is not sufficient for Pom-induced increases in surface markers.
(A) Relative number of live BCBL-1 cells after 48 hours treatment with indicated concentrations of JQ-1 as measured by trypan blue exclusion method. (B) Protein levels of Ikaros, IRF4, cMyc, and control β-actin in the nuclear lysates of BCBL-1 cells treated with various concentrations of JQ-1 for 48 hours. (C) Surface expression levels of ICAM-1 and B7-2 in BCBL-1 cells 48 hours after treatment with JQ-1. Cells were stained with FITC-conjugated IgG isotype control, anti-ICAM-1, or anti-B7-2 antibodies and analyzed using flow cytometry.