Inhibition of DNA pols by aptamers 32 and 45
figureposted on 2011-12-30, 15:59 authored by Leonid V. Gening, Svetlana A. Klincheva, Anastasia Reshetnjak, Arthur P. Grollman, Holly Miller
Taken from "RNA aptamers selected against DNA polymerase β inhibit the polymerase activities of DNA polymerases β and κ"
Nucleic Acids Research 2006;34(9):2579-2586.
Published online 17 May 2006
© The Author 2006. Published by Oxford University Press. All rights reserved. In reactions containing polβ (0.9 nM), polκ (1.7 nM), RB69 pol (1.6 nM) or KF (1.1 nM) and 50 nM 5′-P-labeled primer-template, primer extension was measured in the absence (−) or presence of 300 nM aptamer 32, aptamer 45 or 1000 nM yeast tRNA (T). ( Time course reactions containing polβ (0.3 nM), polκ (0.5 nM) or KF (0.3 nM) and 50 nM 5′-P-labeled primer-template, primer extension was measured in the absence (−) or presence of 1–4 µM aptamer 32 or 1000 nM yeast tRNA.