Inhibition of DNA pols by aptamers 32 and 45

Copyright information:

Taken from "RNA aptamers selected against DNA polymerase β inhibit the polymerase activities of DNA polymerases β and κ"

Nucleic Acids Research 2006;34(9):2579-2586.

Published online 17 May 2006

PMCID:PMC1463896.

© The Author 2006. Published by Oxford University Press. All rights reserved

. In reactions containing polβ (0.9 nM), polκ (1.7 nM), RB69 pol (1.6 nM) or KF (1.1 nM) and 50 nM 5′-P-labeled primer-template, primer extension was measured in the absence (−) or presence of 300 nM aptamer 32, aptamer 45 or 1000 nM yeast tRNA (T). ( Time course reactions containing polβ (0.3 nM), polκ (0.5 nM) or KF (0.3 nM) and 50 nM 5′-P-labeled primer-template, primer extension was measured in the absence (−) or presence of 1–4 µM aptamer 32 or 1000 nM yeast tRNA.