Increased TLR4 expression and microglia activation in the prefrontal cortex, hippocampus and the lumbar region of the spinal cord in DIO mice.
Western blot analysis for TLR4 and Cd11b (microglial marker) in the prefrontal cortex, hippocampus and the lumbar region of the spinal cord. TLR4 is significantly increased in the prefrontal cortex in DIO mice (A), hippocampus (B) and spinal cord (C). Moreover, microglia expression (Cd11b) is significantly increased in DIO in the prefrontal cortex (D) and hippocampus (E), but not in the lumbar region of the spinal cord (F) when compared with lean mice. * p<0.05; ** p<0.01; *** p<0.001; independent sample t-test; data represent mean ± standard error of the mean (SEM) expressed as percentage of the control group.