In vivo inhibition of ClfB-loricrin interaction leads to reduced abscess area and bacterial burden.
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BALB/c mice were infected subcutaneously with 2x107 CFU S. aureus LAC::lux pre-incubated with loricrin loop 2 region (L2v) or GST (24 μM) and abscess lesion size and bacterial burden was measured. Representative lesions from the dorsal area of mice from each group are shown (A) and results are expressed as total lesion size (cm2) ± SEM (B). Bioluminescence imaging was carried out using a Photon Imager and representative in vivo bioluminescence images are shown (C). Results are expressed as mean total photon flux (photons per second per steradian) ± SEM (D). n = 8 per group. Data pooled from 2 independent experiments. Two-way ANOVA with Bonferroni post-test used to analyze differences between groups. * P < 0.05, *** P < 0.001.