In situ GFP expression in immunized zebrafish.
AB fish were immunized with 12 μg of experimental or control vaccine plasmids, followed by electroporation. Seven days post-injection, the successful vaccinations and expression of the antigen-GFP fusion proteins were verified by fluorescence microscopy. The fluorescence resulting from the expression of the antigen-GFP fusion protein is seen in the dorsal muscle near the injection site. For each antigen, a representative example is shown. Non-immunized AB fish were used as a negative control.