In healthy normotensive animals, PVAT-conditioned media does not impact induction of GM-CSF, IFNg, TNFa, IL-4 or IL-10.

Conditioned media was prepared from the mPVAT as described in Methods. Splenocytes were treated with anti-CD3/anti-CD28 (a T cell specific activator) and cultured in PVAT-conditioned media for 96 h. Cytokines were quantified by ELISA (IL-17a) or multiplex bead assay (Luminex for all other cytokines). mPVAT and isolated splenocytes were collected from the same animal. *P<0.05 as compared to CTL diet.

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