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Implementation of a loss-of-function system to determine growth and stress-associated mutagenesis in Bacillus subtilis - Fig 6

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posted on 11.07.2017 by Norberto Villegas-Negrete, Eduardo A. Robleto, Armando Obregón-Herrera, Ronald E. Yasbin, Mario Pedraza-Reyes

Ability of strain B. subtilis PERM1100 (A), PERM1171 (B) and PERM1192 (C) to survive Met-Leu- starvation in selective media supplemented (black bars) or not (white bars) with IPTG. Data were collected from plugs removed from three plates and titers were plated on media containing all essential amino acids every other day for testing of viability of non-revertant background cells (see Materials and Methods for details). Data is represented as the number of CFU per plug. Error bars represent 1 standard error of the mean.