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Impact of Sox2 deletion in epithelial stem/progenitor cells on gene expression in stem/progenitor cells and non-gustatory epithelial cells.

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posted on 15.10.2020, 17:32 by Makoto Ohmoto, Weiwei Lei, Junpei Yamashita, Junji Hirota, Peihua Jiang, Ichiro Matsumoto

A, C: Qualitative histochemical analyses by in situ hybridization of stem/progenitor cell (A) and non-gustatory epithelial cell (C) genes in the CvP of Krt5CreERT2/+; Sox2flox/flox mice (n≥3 at each point) before and after tamoxifen injection. B, D: Quantitative analyses of mRNA expression by PCR of Lgr5 (B, left), Krt5 (B, right), and Sprr2a2 (D) in the CvP of Krt5CreERT2/+; Sox2flox/flox mice before and after tamoxifen injection. Relative gene expression levels were normalized using Gapdh and statistically evaluated by Welch’s t-test (n = 4). Scale bars, 50 μm.

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