Immunostaining of a 2-week Wnt1-Cre;Pdpn^Δ/Δ mouse lower incisor sagittal section for podoplanin (PDPN).

In the hematoxylin-eosin (HE) staining, there are no abnormalities in the bone, dentin, or enamel formation. There is expression of PDPN observed in the epithelial cells: Hertwig’s epithelial root sheath (HS), in the apical bud (Ap), and in the inner enamel epithelial cells (IEE). There is no expression of PDPN observed in the odontoblast layer at the edge of the dental pulp (DP), in the osteocytes of the alveolar bone (AB), or the sheath of the inferior alveolar nerve (Ne). Cross-reaction is observed in the enamel (En) of the labial side but not in the dentin (De), or in the dental pulp fibroblasts (DP), or in the muscle (Mu). Bar: 500μm.