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Immunostaining of “8”-shaped-hatching and U-shaped-hatching blastocysts.

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posted on 06.04.2017 by Yohei Onodera, Kazumasa Takahashi, Mayumi Goto, Mibuki Anzai, Natsuki Ono, Hiromitsu Shirasawa, Wataru Sato, Hiroshi Miura, Naoki Sato, Akira Sato, Yukiyo Kumazawa, Yukihiro Terada

Cdx2 (green) and Oct3/4 (red) were stained with specific antibodies, and nuclei were stained with Hoechst (blue). In the case of “8”-shaped hatching, the figure shows embryos in the Near group (A–D) and Far group (E–H). Whereas the ICM tended to be morphologically compact in the Far group (G), it was scattered in the Near group (C; see Fig 7C).

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