Immunostaining of “8”-shaped-hatching and U-shaped-hatching blastocysts.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Cdx2 (green) and Oct3/4 (red) were stained with specific antibodies, and nuclei were stained with Hoechst (blue). In the case of “8”-shaped hatching, the figure shows embryos in the Near group (A–D) and Far group (E–H). Whereas the ICM tended to be morphologically compact in the Far group (G), it was scattered in the Near group (C; see Fig 7C).