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Immunolocalization of KAPs on WT and APO strains of S. culicis and A. deanei.

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posted on 13.11.2017, 18:27 authored by Silvana Sant´Anna de Souza, Carolina Moura Catta-Preta, João Marcelo P. Alves, Danielle P. Cavalcanti, Marta M. G. Teixeira, Erney P. Camargo, Wanderley De Souza, Rosane Silva, Maria Cristina M. Motta

DAPI was used to label the nucleus and the kinetoplast, and the anti-porin antibody to identify the symbiotic bacterium in WT cells. (A-B): The anti-KAP4 antibody labeled the kinetoplast region that faces the anterior end of A. deanei in WT (A) and APO (B) cells. Note that this labeling in part coincides with DAPI staining. (C-D): For S. culicis, KAP4 labeling is dispersed through the kinetoplast of both strains and overlaps those for DAPI. (E-F): The anti-aKAP23 antibody generated a similar and specific labeling for WT (E) and APO (F) cells of A. deanei. In this case, an overlap with DAPI was not observed indicating that aKAP23 does not co-localize with the kDNA and is probably located at the KFZ. (G-H) Regarding stKAPy, this protein also faces the anterior part of the cell body in both strains, however the labeling is more disperse when compared to that obtained for aKAP23. Arrowheads indicate the kinetoplast. Scale bars equal to 1 μm.