Immunohistochemical analyses of lymph nodes after IR injury.

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posted on 2020-10-06, 17:27 authored by Florian S. Frueh, Bijan Jelvani, Claudia Scheuer, Christina Körbel, Bong-Sung Kim, Pietro Giovanoli, Nicole Lindenblatt, Yves Harder, Emmanuel Ampofo, Michael D. Menger, Matthias W. Laschke

A-D Immunohistochemical detection of HO-1⁺ cells (arrowheads) in control (A), IR-45 (B) and IR-120 (C) lymph nodes. D Quantitative analysis of HO-1⁺ cells (%). E-H Immunohistochemical detection of PCNA⁺ cells (arrowheads) in control (E), IR-45 (F) and IR-120 (G) lymph nodes. H Quantitative analysis of PCNA⁺ cells (%). I-L Immunohistochemical detection of Casp-3⁺ cells (arrowheads) in control (I), IR-45 (J) and IR-120 (K) lymph nodes. L Quantitative analysis of Casp-3⁺ cells (%). M-P Immunohistochemical detection of CD68⁺ macrophages (arrowheads) in control (M), IR-45 (N) and IR-120 (O) lymph nodes. P Quantitative analysis of CD68⁺ cells (mm^-2). Q-T Immunohistochemical detection of MPO⁺ neutrophilic granulocytes (arrowheads) in control (Q), IR-45 (R) and IR-120 (S) lymph nodes. T Quantitative analysis of MPO⁺ cells (mm^-2). Mean ± SEM, n = 6–8, *P < 0.05, **P < 0.001. Scale bars: A-C, E-G and I-K = 25 μm, M-O and Q-S = 50 μm. Casp-3 = cleaved caspase-3, HO = heme oxygenase, IR = ischemia/reperfusion, MPO = myeloperoxidase, PCNA = proliferating cell nuclear antigen, VLN = vascularized lymph node.