pone.0216926.g005.tif (3.07 MB)
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Immunofluorescence (CD68) results of high-intensity focused ultrasound (HIFU) treatment after silicone injection.

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posted on 14.05.2019, 17:34 authored by Joon Seok, Soo Hyun Woo, Tae Rin Kwon, Jong Hwan Kim, Guk Jin Jeong, Kapsok Li, Woo Seob Kim, Beom Joon Kim

(a) The first row depicts the immunofluorescence (IF) of the monoclonal antibodies against CD68. The second row depicts the results of staining with 4′,6-diamidino-2-phenylindole (DAPI), and the third row depicts the images of the CD68 and DAPI signals (×100). The first column shows IF staining of CD68 in the control group (untreated) on Day 7. There was minimal activation of macrophages in the control group. The second column shows IF staining in the experimental group (treated with HIFU) on Day 4. Increased IF staining reveals the activation of macrophages. The third column shows the IF staining of the experimental group on Day 7. There was an even greater level of macrophage activation. The fourth column depicts IF staining in the experimental group on Day 30, revealing reduced activity of the macrophages. (b) Expression of CD68 stained by IF (per/field). Markers for the CD68 antigen are most frequently used for the identification of macrophages. (**P < 0.01; ***P < 0.001 vs. the Silicone only group. N = 5).