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Immunocytochemical detection of endogenous hFAM76B.

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posted on 28.03.2016, 05:57 by Xiaojing Zheng, Yanqing Li, Junli Zhao, Dongyang Wang, Haibin Xia, Qinwen Mao

HepG2 and Shsy5y FAM76B-/- HEK 293 cells were fixed with 4% paraformaldehyde and then stained with anti-FAM76B MAbs. Normal mouse serum was used as a negative control. Primary Abs were detected by a Vectastain ABC Kit with DAB as a substrate. Nuclei were labeled with hematoxylin. Scale bar = 50μm.