Immunocytochemical detection of endogenous hFAM76B.
HepG2 and Shsy5y FAM76B-/- HEK 293 cells were fixed with 4% paraformaldehyde and then stained with anti-FAM76B MAbs. Normal mouse serum was used as a negative control. Primary Abs were detected by a Vectastain ABC Kit with DAB as a substrate. Nuclei were labeled with hematoxylin. Scale bar = 50μm.