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Immunocytochemical characterisation of Synapsin I expression in differentiating cells.

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posted on 04.05.2016 by Kamila Rosiak, Maciej Smolarz, Wojciech J. Stec, Joanna Peciak, Dawid Grzela, Marta Winiecka-Klimek, Ewelina Stoczynska-Fidelus, Barbara Krynska, Sylwester Piaskowski, Piotr Rieske

Synapsin I expression after 14 days of differentiation in ebiNSc cultures expressing IDH1R132H compared to control cultures. (A) Synapsin I (SYN1) expression in ebiNSc. (B) Synapsin I expression in ebiNScIDH1wt. (C) Synapsin I (SYN1) expression in ebiNScempty (D) Staining with antibodies to Synapsin I (green) and IDH1R132H (red) in ebiNScIDH1R132H. No SYN1-positive cells are visible. All images at magnification 200x, scale bars mark 100μm. (E) Graphs demonstrating the percentage of SYN1-positive cells after 14 days of differentiation in ebiNSc expressing IDH1R132H and control cultures. Error bars indicate SEM. Statistical significance calculated by Kruskal-Wallis with Dunn’s multiple comparison test. *, p<0.05; **, p<0.01; ***, p<0.005. (F) Graph demonstrating SYN1 expression at the mRNA level after 0, 7 and 14 days of differentiation (d0, d7, d14) in ebiNSc expressing IDH1R132H and control cultures. Error bars indicate SEM. Statistical significance calculated by Two-way ANOVA with Bonferroni’s post-comparison test. **, p<0.01; ***, p<0.005; ns, not significant.

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