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Immunoblotting analysis of various αS antibodies that react with mouse αS using total brain lysates.

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posted on 14.09.2017, 17:53 by Jess-Karan S. Dhillon, Cara Riffe, Brenda D. Moore, Yong Ran, Paramita Chakrabarty, Todd E. Golde, Benoit I. Giasson

20 μg of total brain homogenates from αS KO and nTG mice were resolved on 15% polyacrylamide gels and analyzed as described in “Materials and Methods”. Immunoblot analysis was performed with each antibody indicated above. The arrows indicates αS. The mobilities of molecular mass markers are indicated on the left. Both αS KO and nTG mice are of the same genetic background as experimental synucleinopathy models, C57BL6/C3H.