Immunoblotting analysis of epitope reactivity of N-terminal anti-αS antibodies using recombinant mouse αS, human αS, and human βS.
100 ng of each recombinant protein were loaded on 15% polyacrylamide gels and analyzed as described in “Material and Methods”. Immunoblot analysis was performed with each antibody indicated above. The mobilities of molecular mass markers are indicated on the left. Mα = Mouse αS, Hα = Human αS, Hβ = Human βS.