EutQ-QII-Opt1-B5-280211.jpg (76.83 kB)

Image of a CD1925 protein crystal.

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posted on 28.02.2013, 10:05 by Jon Marles-WrightJon Marles-Wright

Image of Clostridium difficile CD1925, EutQ, protein crystal.

 

Methods

CD1925(17–157)-His was concentrated to 8 mg/ml in buffer A and crystallised by hanging drop vapour diffusion in drops of 1 µl protein plus 1 µl crystallisation solution over 1 ml of the latter. Crystals were obtained in 100 mM sodium acetate pH 4.5, 35% (w/v) PEG 6000, 200 mM MgCl2, these were harvested by transfer to a cryoprotection solution containing the well solution supplemented with 20% v/v PEG300 and flash cooled in liquid nitrogen. All crystallographic data were collected on beamlines I02 and I04 at Diamond Light Source (Didcot, UK) at 100 K using ADSC CCD detectors.

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