Image_1_Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T Cells.pdf (320.3 kB)

Image_1_Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T Cells.pdf

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posted on 25.06.2020 by Alexandre Juillerat, Diane Tkach, Ming Yang, Alex Boyne, Julien Valton, Laurent Poirot, Philippe Duchateau

Here, we developed a straightforward methodology to generate TCRαβ negative (allogeneic) cells for CAR-T cell therapies. With an early and transient expression of an anti-CD3 CAR in the engineered donor T cells, we programmed these cells to self-eliminate the TCR+ cell population and obtained an ultrapure TCRαβ population (99–99.9%) at the end of the CAR-T production. This novel and easy-to-implement procedure preserves the production yield and cell fitness and has the potential to streamline the manufacturing of “off-the-shelf” CAR T-cell therapies.

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