Identification of <i>pfap2-g</i> transcription start sites (TSSs).

<p>Position relative to the start codon and frequency (number of clones) of the TSSs identified by the 5’RACE analysis of <i>pfap2-g</i> in the E5 and E5-AP2-G-DD (E5-DD) lines. The E5-DD line was cultured in the presence of Shld1. TSSs were identified from experiments in which PCR was performed with either LA Taq or KAPA DNA polymerases, as indicated. The position of the two main TSS blocks (B1 and B2) and the region containing the previously described PfAP2-G binding motifs is indicated in the schematic (to scale) below.</p>