Identification of double-stranded RNA-binding defective mutants of σ3.
(A) Space-filling surface view of the σ3 dimer structure (PDB ID: 1FN9) showing mutagenized positively charged residues (red and gold). Red indicates residues that disrupted the dsRNA-binding capacity of σ3. Gold indicates residues that retain their capacity to bind to dsRNA. Residues labelled in gray indicate those express poorly. The σ3 monomers are shown in different shades of gray. (B) Pull-down assay showing capacity of σ3, but not σNS to bind poly I:C. (C and D) Poly(I:C) pull-down assay showing capacity of σ3 mutants to bind dsRNA and relative expression levels when proteins were expressed exogenously. Findings are summarized in the table. An asterisk (*) and gray indicate mutants that express poorly. Red indicates residues that disrupted the dsRNA-binding capacity of σ3.