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Identification and characterization of the Fasciola hepatica sodium- and chloride-dependent taurine transporter - Fig 7

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posted on 27.04.2018, 17:35 by Bulut Hamali, Sandra Pichler, Elisabeth Wischnitzki, Klaus Schicker, Melanie Burger, Marion Holy, Kathrin Jaentsch, Martina Molin, Eva Maria Sehr, Oliver Kudlacek, Michael Freissmuth

Detection by immunoblotting of the F. hepatica taurine transporter after heterologous expression (A, B) and in lysates prepared from adult flukes (C). A&B: lysates (20 μg/lane) were prepared from untransfected HEK293 cells (lane 1), from HEK293 cells stably expressing the F. hepatica taurine transporter (lane 2) and a YFP-tagged version of the transporter (lane 3). After electrophoretic separation on an SDS-polyacrylamide gel, the proteins were transferred to a nitrocellulose membrane, which was probed with an affinity purified rabbit polyclonal antibody raised against the N-terminus (A) and an anti-GFP antibody (B). C: lysates (1 μg) prepared from untransfected HEK293 cells (lane 1) or from HEK293 expressing the untagged (lane 2) and the YFP-tagged F. hepatica taurine transporter (lane 3) were resolved by denaturing gel electrophoresis together with detergent extracts (40 μg) of adult fluke homogenates (lane 4) and of tegumental particulate material (lane 5). After transfer to nitrocellulose membranes, the immunoreactive bands were visualized as described for panels A & B. The immunoblots are representative of four independent experiments.