IC-MS/MS analysis of intracellular metabolites of the Δfbpase mutant incubated with [U-13C]-proline.
figure
posted on 2018-12-17, 19:00 authored by Marion Wargnies, Eloïse Bertiaux, Edern Cahoreau, Nicole Ziebart, Aline Crouzols, Pauline Morand, Marc Biran, Stefan Allmann, Jane Hubert, Oriana Villafraz, Yoann Millerioux, Nicolas Plazolles, Corinne Asencio, Loïc Rivière, Brice Rotureau, Michael Boshart, Jean-Charles Portais, Frédéric BringaudThe parental (EATRO1125.T7T), Δfbpase and Δfbpase/FBPase.i cell lines were incubated for 2 h in PBS containing 2 mM [U-13C]-proline. Enrichment of key glycolytic intermediates at carbon positions 0 to 6 (m0 to m6) is expressed as percentage of all the corresponding molecules. For abbreviations, see Figs 1 and 2.
History
Usage metrics
Categories
Keywords
differentiationprocyclicfructose 6- phosphateenzymeΔ fbpasemammalian-infective metacyclic parasites13 C-enriched prolineppdkvectortrypanosomeglycerol conversion pathwaybruceipepckfeedPEPglucose-free conditionsΔ fbpase cell linepyruvate phosphate dikinasemetabolismincorporationglucose 6- phosphategluconeogenesiRNA-binding protein 6 over-expressionPPDKtsetseG 6Pglycerol conversion pathwaysepimastigotePEPCKcell line re-expressing FBPaseG 6P production
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC