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Download fileIC-MS/MS analysis of intracellular metabolites of the Δfbpase mutant incubated with [U-13C]-proline.
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posted on 17.12.2018, 19:00 authored by Marion Wargnies, Eloïse Bertiaux, Edern Cahoreau, Nicole Ziebart, Aline Crouzols, Pauline Morand, Marc Biran, Stefan Allmann, Jane Hubert, Oriana Villafraz, Yoann Millerioux, Nicolas Plazolles, Corinne Asencio, Loïc Rivière, Brice Rotureau, Michael Boshart, Jean-Charles Portais, Frédéric BringaudThe parental (EATRO1125.T7T), Δfbpase and Δfbpase/FBPase.i cell lines were incubated for 2 h in PBS containing 2 mM [U-13C]-proline. Enrichment of key glycolytic intermediates at carbon positions 0 to 6 (m0 to m6) is expressed as percentage of all the corresponding molecules. For abbreviations, see Figs 1 and 2.
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differentiationprocyclicfructose 6- phosphateenzymeΔ fbpasemammalian-infective metacyclic parasites13 C-enriched prolineppdkvectortrypanosomeglycerol conversion pathwaybruceipepckfeedPEPglucose-free conditionsΔ fbpase cell linepyruvate phosphate dikinasemetabolismincorporationglucose 6- phosphategluconeogenesiRNA-binding protein 6 over-expressionPPDKtsetseG 6Pglycerol conversion pathwaysepimastigotePEPCKcell line re-expressing FBPaseG 6P production
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