pone.0222093.g003.tif (3.39 MB)

Hypoxically preconditioned secretome of BMSCs from SCD patients presents enrichment of angiogenic factors.

Download (3.39 MB)
posted on 2019-10-30, 17:24 authored by Tiago O. Ribeiro, Brysa M. Silveira, Mercia C. Meira, Ana C. O. Carreira, Mari Cleide Sogayar, Roberto Meyer, Vitor Fortuna

(A) Differential secretion of trophic factors in conditioned medium during 48h of hypoxic (right) or normoxic (left) culturing. Representative images of membrane-based antibody arrays are shown. Dashed black boxes indicate positive internal control areas. (B) Relative expression levels of secreted bioactive factors categorized according to primary function. Quantification of mean spot pixel density was normalized to reference spots. (C) Relative expression of genes related to angiogenesis and tissue repair in BMSCs from SCD patients after culturing for 48hs under normoxic or hypoxic conditions. Total RNA was isolated and expression levels were measured by quantitative real time RT-PCR. Representative data from three independent experiments are shown as means ± SD. Asterisks correspond to statistical analysis comparisons of each condition (*p < 0.05; **p < 0.01).