Hypoxia exposure upregulated miR-210-3p via transcriptional activity of HIF-1α.
A. After hypoxia or CoCl2 exposure for 24 and 48 h, the expressing levels of miR-9 and miR-210-3p were evaluated by RT-qPCR. *P<0.05, vs. 24h normoxia group; #P<0.05, vs. 48h normoxia group. Square represents miR-210-3p/CoCl2 group; Triangle represents miR-9/hypoxia group; reverse triangle represents miR-9/CoCl2 group; circle represents miR-210-3p/hypoxia group. B. After co-treatment with 1 ng/ml echinomycin, the expressing levels of miR-9 and miR-210-3p in glioma cells were detected. *P<0.05, vs. hypoxia group; #P<0.05, vs. CoCl2 group. C. After echinomycin treatment or introduction of miR-210 for 24 or 48 h, cell viability was measured by performing CCK-8. *P<0.05, vs. hypoxia+echinomycin group; #P<0.05, vs. CoCl2+echinomycin group.