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Hypoxia causes reversible neuronal cilia resorption.

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posted on 01.08.2019, 17:41 by Brian K. A. Choi, Philippe M. D’Onofrio, Alireza P. Shabanzadeh, Paulo D. Koeberle

(A and B) Normal N1E-115 cells (Left A) and HT22 cultures (Left B) exhibited primary cilia (arrowheads in A) demonstrated by immunofluorescence against Actub. Five hours of hypoxia (middle) resulted in the loss of primary cilia and diffuse staining of Actub in both cell lines (Middle panel). Following hypoxia, 24 hours of normoxia returned the expression of primary cilia in both cell lines (Right). N1E-115 Scale bar = 25 μm, HT22 Scale bar = 10 μm. (C) Quantification of N1E-115 cell survival by WST-8 assay showing increased metabolic activity at 5 hours of hypoxia that normalized after 24 hours of normoxia. Data presented as mean ± SEM relative to normal. ** p<0.01.