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Homology recognition and ensuing mutation are not coupled with respect to the involved base-pairs.

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posted on 05.05.2016, 07:54 by Eugene Gladyshev, Nancy Kleckner

(a) RIP mutation profiles of the two diagrammed repeat constructs are compared along their entire lengths. A 200-bp portion of the reference sequence that appears to have similar levels of RIP in both cases is outlined. We note that a distal portion of the same reference sequence (marked with “*”) escapes RIP in the case of 4H-7N_1 but not in the case of 4H-7N_7. (b) RIP mutation profiles along the 200-bp portion of the reference sequence at the base-pair resolution. “●●●●” indicates the positions, in the reference sequence, of the 4-bp homologous units defined by the corresponding test sequences 4H-7N_1 (upper profile) and 4H-7N_7 (lower profile). (c) Correlation of mutations observed for individual sites within the 200-bp segment (Panel b). r is the Pearson correlation coefficient.