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Histone methyltransferase activity of SUVR proteins

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posted on 30.12.2011, 16:43 by Tage Thorstensen, Andreas Fischer, Silje V. Sandvik, Sylvia S. Johnsen, Paul E. Grini, Gunter Reuter, Reidunn B. Aalen

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Taken from "The SUVR4 protein is a nucleolar histone methyltransferase with preference for monomethylated H3K9"

Nucleic Acids Research 2006;34(19):5461-5470.

Published online 04 Oct 2006

PMCID:PMC1636477.

© 2006 The Author(s)

() Assay for GST-SUVR1 (amino acid 332–688), GST-SUVR2 (amino acid 348–697) and the positive control GST-SUV39H1 (amino acid 82–412) on calf thymus histones (C). () Assay for the full-length SUVR4a GST-fusion protein (1–465 amino acid) and GST-SUV39H1 (amino acid 82–412) on calf thymus histones (C) or recombinant histones (R). Upper panels in (A) and (B) show Coomassie stained SDS–PAGE gel with GST-fusion proteins of expected size indicated with an asterisk. Lower panels show fluorograms with the specific localization of the transferred C labelled methyl groups. () Fluorogram of assay for GST-SUVR4 on histone H3 peptides residues 1–20 unmodified, monomethylated or dimethylated at K9. () Western analysis using a diMeH3K9 antibody after incubation of monomethylated H3K9 peptides with GST-SUVR4 and as a control SU(VAR)3-9.

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