Hindlimb cells are more adhesive in vivo and in vitro than forelimb cells.

A) Enzymatic dissociation of 11.5dpc limb buds with dispase releases more hindlimb cells than forelimb cells (p = 0.0021, n = 6). B) When micromass cultures are challenged after 1 hour of adhesion to the cell culture substrate, fewer hindlimb cells are left in the culture than forelimb cells (p = 0.011, n = 4 pools of >4 limbs each, normalised for limb number). *p<0.05, **p<0.01, ***p<0.001, Student’s t-test. C–K; Cell mixing experiments, 1:1 mix of Prx1eGFP hindlimb cells/wild type forelimb cells (C–E), Prx1-eGFP forelimb/wild type forelimb (F–H) and Prx1-eGFP hindlimb/wild type hindlimb (I–K) cells were stained for GFP protein (green) and nuclei (blue, DAPI).