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HTLV-1 infection in neutrophils and monocytes.

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posted on 29.11.2017, 18:39 by Rie Furuta, Jun-ichirou Yasunaga, Michi Miura, Kenji Sugata, Akatsuki Saito, Hirofumi Akari, Takaharu Ueno, Norihiro Takenouchi, Jun-ichi Fujisawa, Ki-Ryang Koh, Yusuke Higuchi, Mohamed Mahgoub, Masakazu Shimizu, Fumihiko Matsuda, Anat Melamed, Charles R. Bangham, Masao Matsuoka

(A) Tax expression is detected by confocal immunofluorescence microscopy in MT4 and neutrophils from HAM/TSP patients using anti-Tax antibody and secondary Alexa Fluor 488 antibody. Myeloperoxidase expression is also detected in neutrophils using anti-MPO antibody and secondary Alexa Fluor 568 antibody. Jurkat was used as a negative control. MT4, HTLV-1 infected cell line; Jurkat cells, HTLV-1 negative human T-cell line. DAPI (blue) was used for nuclei staining. (B) JET WT35 cells were co-cultured with HPB-ATL-2 cells in the presence and absence of azidothymidine (AZT) or raltegravir (RAL), or HTLV-1 uninfected T cell line, CCRF-CEM. The number of tdTomato-positive cells is presented. (C) Dendritic cells were induced from monocytes from HAM/TSP patients, and then differentiated DCs were co-cultured with JET WT35. After 48 hours, newly infected JET WT35 was shown to be red. (D) Quantitative data of co-culture between differentiated DCs and JET WT35 cells. The count of tdTomato positive cells and the number of cells used for this experiment are shown.