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Guanosine has no effect on heme-oxygenase-2 protein expression in PC12 cells

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posted on 2011-12-31, 10:32 authored by Christian Bau, Pamela J. Middlemiss, Shaun Hindley, Shucui Jiang, Renata Ciccarelli, Francesco Caciagli, Patrizia DiIorio, Eva S. Werstiuk, Michel P. Rathbone

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Taken from "Guanosine stimulates neurite outgrowth in PC12 cells via activation of heme oxygenase and cyclic GMP"

Purinergic Signalling 2005;1(2):161-172.

Published online Jan 2005


PC12 cells were cultured on plates coated with poly--ornithine for 72 h. Cells were then grown in serum-reduced medium (3% heat-inactivated fetal calf serum and 3% heat-inactivated horse serum) for 12 h, and were treated with guanosine (G, 300 µM) or NGF (N, 40 ng/ml) or guanosine (300 µM) plus NGF (40 ng/ml) (G + N), or with no added treatments (C) as described in Figure . The expression of heme oxygenase-2 was determined at various time points (6, 12, 24 and 48 h) by Western immunoblot analysis. Immunoblots were quantified by densitometric analysis and were normalized to the corresponding β-actin bands as described in the Materials and methods. Open bars: untreated controls (C), closed bars: guanosine plus NGF (G + N) treatment, stippled bars: NGF (N) treatment, hatched bars: guanosine (G) treatment. Statistical analysis was performed using a two-way ANOVA. (a) Data represent the mean optical density T SEM obtained in three independent experiments. (b) Results are representative Western immunoblots obtained in these experiments.


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