11302_2005_Article_6214_Fig5.jpg (108.68 kB)
Guanosine has no effect on heme-oxygenase-2 protein expression in PC12 cells
figureposted on 2011-12-31, 10:32 authored by Christian Bau, Pamela J. Middlemiss, Shaun Hindley, Shucui Jiang, Renata Ciccarelli, Francesco Caciagli, Patrizia DiIorio, Eva S. Werstiuk, Michel P. Rathbone
Taken from "Guanosine stimulates neurite outgrowth in PC12 cells via activation of heme oxygenase and cyclic GMP"
Purinergic Signalling 2005;1(2):161-172.
Published online Jan 2005
PMCID:PMC2096532.PC12 cells were cultured on plates coated with poly--ornithine for 72 h. Cells were then grown in serum-reduced medium (3% heat-inactivated fetal calf serum and 3% heat-inactivated horse serum) for 12 h, and were treated with guanosine (G, 300 µM) or NGF (N, 40 ng/ml) or guanosine (300 µM) plus NGF (40 ng/ml) (G + N), or with no added treatments (C) as described in Figure . The expression of heme oxygenase-2 was determined at various time points (6, 12, 24 and 48 h) by Western immunoblot analysis. Immunoblots were quantified by densitometric analysis and were normalized to the corresponding β-actin bands as described in the Materials and methods. Open bars: untreated controls (C), closed bars: guanosine plus NGF (G + N) treatment, stippled bars: NGF (N) treatment, hatched bars: guanosine (G) treatment. Statistical analysis was performed using a two-way ANOVA. (a) Data represent the mean optical density T SEM obtained in three independent experiments. (b) Results are representative Western immunoblots obtained in these experiments.