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Growth factor and NOS expression of VLN flaps after IR injury.

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posted on 06.10.2020, 17:27 by Florian S. Frueh, Bijan Jelvani, Claudia Scheuer, Christina Körbel, Bong-Sung Kim, Pietro Giovanoli, Nicole Lindenblatt, Yves Harder, Emmanuel Ampofo, Michael D. Menger, Matthias W. Laschke

A-D Western blots (A and B) with quantification (C and D) of VEGF-C, VEGF-D and VEGF-A expression of control (white), IR-45 (cyan) and IR-120 (red) VLN flaps. Bands were cropped from different parts of the same gel. Panels A and C represent analyses of lymph nodes and panels B and D analyses of adipose tissue. Mean ± SEM, n = 4, *P < 0.05, **P < 0.001, n.s. = not significant. E-J Western blots (E and F) with quantification of iNOS (G and I) and p-eNOS/eNOS (H and J) expression of control (white), IR-45 (cyan) and IR-120 (red) VLN flaps. Bands were cropped from different parts of the same gel. Panels E, G and H represent analyses of lymph nodes and panels F, I and J analyses of adipose tissue. Mean ± SEM, n = 4, *P < 0.05, n.s. = not significant. IR = ischemia/reperfusion, iNOS = inducible nitric oxide synthase (NOS), eNOS = endothelial NOS, p-eNOS = phospho-eNOS, VEGF = vascular endothelial growth factor, VLN = vascularized lymph node.

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