pone.0239517.g005.tif (511.05 kB)

Growth factor and NOS expression of VLN flaps after IR injury.

Download (511.05 kB)
figure
posted on 06.10.2020 by Florian S. Frueh, Bijan Jelvani, Claudia Scheuer, Christina Körbel, Bong-Sung Kim, Pietro Giovanoli, Nicole Lindenblatt, Yves Harder, Emmanuel Ampofo, Michael D. Menger, Matthias W. Laschke

A-D Western blots (A and B) with quantification (C and D) of VEGF-C, VEGF-D and VEGF-A expression of control (white), IR-45 (cyan) and IR-120 (red) VLN flaps. Bands were cropped from different parts of the same gel. Panels A and C represent analyses of lymph nodes and panels B and D analyses of adipose tissue. Mean ± SEM, n = 4, *P < 0.05, **P < 0.001, n.s. = not significant. E-J Western blots (E and F) with quantification of iNOS (G and I) and p-eNOS/eNOS (H and J) expression of control (white), IR-45 (cyan) and IR-120 (red) VLN flaps. Bands were cropped from different parts of the same gel. Panels E, G and H represent analyses of lymph nodes and panels F, I and J analyses of adipose tissue. Mean ± SEM, n = 4, *P < 0.05, n.s. = not significant. IR = ischemia/reperfusion, iNOS = inducible nitric oxide synthase (NOS), eNOS = endothelial NOS, p-eNOS = phospho-eNOS, VEGF = vascular endothelial growth factor, VLN = vascularized lymph node.

History

Licence

Exports