Growth factor and NOS expression of VLN flaps after IR injury.

<p><b>A-D</b> Western blots (<b>A</b> and <b>B</b>) with quantification (<b>C</b> and <b>D</b>) of VEGF-C, VEGF-D and VEGF-A expression of control (white), IR-45 (cyan) and IR-120 (red) VLN flaps. Bands were cropped from different parts of the same gel. Panels <b>A</b> and <b>C</b> represent analyses of lymph nodes and panels <b>B</b> and <b>D</b> analyses of adipose tissue. Mean ± SEM, n = 4, *<i>P</i> < 0.05, **<i>P</i> < 0.001, n.s. = not significant. <b>E-J</b> Western blots (<b>E</b> and <b>F</b>) with quantification of iNOS (<b>G</b> and <b>I</b>) and p-eNOS/eNOS (<b>H</b> and <b>J</b>) expression of control (white), IR-45 (cyan) and IR-120 (red) VLN flaps. Bands were cropped from different parts of the same gel. Panels <b>E, G</b> and <b>H</b> represent analyses of lymph nodes and panels <b>F, I</b> and <b>J</b> analyses of adipose tissue. Mean ± SEM, n = 4, *<i>P</i> < 0.05, n.s. = not significant. IR = ischemia/reperfusion, iNOS = inducible nitric oxide synthase (NOS), eNOS = endothelial NOS, p-eNOS = phospho-eNOS, VEGF = vascular endothelial growth factor, VLN = vascularized lymph node.</p>