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Graphic comparison of HDR efficiency of donor E.

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posted on 2021-03-05, 18:27 authored by Mariateresa Di Stazio, Nicola Foschi, Emmanouil Athanasakis, Paolo Gasparini, Adamo Pio d’Adamo

The triple transfection and nocodazole treatment increased the HDR efficiency by about two-fold. StUns, single transfection in unsynchronized cells; StSyn, single transfection in synchronized cells; TtUns, triple transfection in unsynchronized cells; TtSyn, triple transfection in synchronized cells.

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Systematic analysis of factors that improve homologous direct repair (HDR) efficiency in CRISPR/Cas9 technique
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cell synchronization cycleNMsingle-stranded oligo DNA nucleotidesHDR efficiencyCRISPRTNF

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