Graded increase of eGFP expression of pRTS-1 transfected TGR-1 cells with increasing Dox concentration
figureposted on 2011-12-31, 00:12 authored by Georg W. Bornkamm, Christian Berens, Conny Kuklik-Roos, Jean-Marie Bechet, Gerhard Laux, Jürgen Bachl, Martin Korndoerfer, Martin Schlee, Michael Hölzel, Anastassia Malamoussi, Rob D. Chapman, Falk Nimmerjahn, Josef Mautner, Wolfgang Hillen, Hermann Bujard, Jean Feuillard
Taken from "Stringent doxycycline-dependent control of gene activities using an episomal one-vector system"
Nucleic Acids Research 2005;33(16):e137-e137.
Published online 7 Sep 2005
© The Author 2005. Published by Oxford University Press. All rights reservedTGR-1 cells transfected with pRTS-1 were plated in six-well plates and different concentrations of Dox added (). The numbers depicted in the histograms represent ng/ml Dox. Mean fluorescence of eGFP expression was plotted versus the Dox concentration in a double logarithmic plot (). Note that, although the peak of eGFP expressing cells is very broad at intermediate Dox concentrations (25–100 ng/ml), induction does not follow an ‘all or none’ pattern.