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Graded increase of eGFP expression of pRTS-1 transfected TGR-1 cells with increasing Dox concentration

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posted on 31.12.2011, 00:12 by Georg W. Bornkamm, Christian Berens, Conny Kuklik-Roos, Jean-Marie Bechet, Gerhard Laux, Jürgen Bachl, Martin Korndoerfer, Martin Schlee, Michael Hölzel, Anastassia Malamoussi, Rob D. Chapman, Falk Nimmerjahn, Josef Mautner, Wolfgang Hillen, Hermann Bujard, Jean Feuillard

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Taken from "Stringent doxycycline-dependent control of gene activities using an episomal one-vector system"

Nucleic Acids Research 2005;33(16):e137-e137.

Published online 7 Sep 2005

PMCID:PMC1201338.

© The Author 2005. Published by Oxford University Press. All rights reserved

TGR-1 cells transfected with pRTS-1 were plated in six-well plates and different concentrations of Dox added (). The numbers depicted in the histograms represent ng/ml Dox. Mean fluorescence of eGFP expression was plotted versus the Dox concentration in a double logarithmic plot (). Note that, although the peak of eGFP expressing cells is very broad at intermediate Dox concentrations (25–100 ng/ml), induction does not follow an ‘all or none’ pattern.

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