Gni137f6.jpg (42.79 kB)
Download file

Graded increase of eGFP expression of pRTS-1 transfected TGR-1 cells with increasing Dox concentration

Download (0 kB)
posted on 2011-12-31, 00:12 authored by Georg W. Bornkamm, Christian Berens, Conny Kuklik-Roos, Jean-Marie Bechet, Gerhard Laux, Jürgen Bachl, Martin Korndoerfer, Martin Schlee, Michael Hölzel, Anastassia Malamoussi, Rob D. Chapman, Falk Nimmerjahn, Josef Mautner, Wolfgang Hillen, Hermann Bujard, Jean Feuillard

Copyright information:

Taken from "Stringent doxycycline-dependent control of gene activities using an episomal one-vector system"

Nucleic Acids Research 2005;33(16):e137-e137.

Published online 7 Sep 2005


© The Author 2005. Published by Oxford University Press. All rights reserved

TGR-1 cells transfected with pRTS-1 were plated in six-well plates and different concentrations of Dox added (). The numbers depicted in the histograms represent ng/ml Dox. Mean fluorescence of eGFP expression was plotted versus the Dox concentration in a double logarithmic plot (). Note that, although the peak of eGFP expressing cells is very broad at intermediate Dox concentrations (25–100 ng/ml), induction does not follow an ‘all or none’ pattern.