Graded increase of eGFP expression of pRTS-1 transfected TGR-1 cells with increasing Dox concentration
Taken from "Stringent doxycycline-dependent control of gene activities using an episomal one-vector system"
Nucleic Acids Research 2005;33(16):e137-e137.
Published online 7 Sep 2005
© The Author 2005. Published by Oxford University Press. All rights reservedTGR-1 cells transfected with pRTS-1 were plated in six-well plates and different concentrations of Dox added (). The numbers depicted in the histograms represent ng/ml Dox. Mean fluorescence of eGFP expression was plotted versus the Dox concentration in a double logarithmic plot (). Note that, although the peak of eGFP expressing cells is very broad at intermediate Dox concentrations (25–100 ng/ml), induction does not follow an ‘all or none’ pattern.