Gossypol prevents collagen gene expression.
Mice were exposed to bleomycin and treated with gossypol as indicated in Fig 2 and sacrificed on day 7. Collagen 1α1 and collagen 3α1 gene expression were measured by qRT-PCR. Data are displayed as fold changes from saline and vehicle treated controls normalized to 18S. Collagen 1α1 mRNA was measured on day 7 (A) and day 21 (B). Collagen 3α1 mRNA was measured on day 7(C) and day 21 (D). Data are displayed as mean ± SEM. *p≤0.05 by ANOVA. n = 3–9 mice per group.