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Genotyping results for PrASE (blue upper panels) and ASE (red lower panels)

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posted on 2011-12-30, 23:51 authored by Emilie Hultin, Max Käller, Afshin Ahmadian, Joakim Lundeberg

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Taken from "Competitive enzymatic reaction to control allele-specific extensions"

Nucleic Acids Research 2005;33(5):e48-e48.

Published online 14 Mar 2005


© The Author 2005. Published by Oxford University Press. All rights reserved

The 36 analyzed samples are visualized in 13 cluster diagrams for each SNP. The -axes represent allelic fractions that are calculated by the equation spot1/(spot1 + spot2), where spot1 and spot2 correspond to fluorescent signal intensity from primer extension of the first and the second allele, respectively. The -axes represent logarithmic value of the total fluorescent signal intensity. Circles indicate the different genotypes, where samples scored as heterozygous are situated in the middle circle with an optimal allelic fraction close to 0.5. Homozygous samples for the first allele and the second allele are located in the circles with allelic fractions close to 1 and 0, respectively. Note that, for SNP 10, the scoring with ASE is impossible, while PrASE generates distinct clusters.


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