Genomic variants are selected in ulp2Δ/Δ cells challenged with additional stress.

(A) Schematic of experimental design. The FLC-1 ulp2Δ/Δ isolate was selected from a casitone agar plate containing 128 μg/ml fluconazole (FLC) passaged two times (2X) non-selective (N/S) agar plates and its genome sequenced by Illumina technology. FLC-2, FLC-3 and FLC-4 ulp2Δ/Δ isolates were selected from a casitone agar plate containing 128 μg/ml fluconazole (FLC) and the genome was sequenced by Illumina technology. (B) Serial dilution assay of ulp2Δ/Δ parental (P) and fluconazole-recovered isolates (FLC-1) in unstressed (N/S) or stress (UV, MMS, HU, H2O2 and 39°C) growth conditions. (C) Left: Representative images displaying colony morphologies of ulp2Δ/Δ parental (P) and fluconazole-recovered isolates (FLC-1). Right: Quantification (%) of smooth and wrinkled colonies in WT and ulp2Δ/Δ strains (D) Left: Representative images displaying the morphologies of ulp2Δ/Δ parental (P) and fluconazole-recovered isolates (FLC-1). Right: Quantification (%) of yeast and filamentous morphologies in WT and ulp2Δ/Δ strains. Error bar: Standard deviation of 3 biological replicates (E) Whole genome sequencing data for four single colonies isolated from 128 μg/ml fluconazole plates (FLC1-FLC4). The chromosome copy number is plotted along the y-axis (1–4 copies).