Gene expression dynamics for pluripotency-, mesoderm- and cardiac lineage-associated transcription factors in cardiac-differentiated Nkx2.5 EmGFP ES cells.

A) pluripotency markers Nanog, Oct4 and Sox2, B) mesodermal marker Brachyury T and early cardiac lineage transcription factors Mesp1, Nkx2.5, Tbx5 and Isl1, C) cardiac-specific contractile proteins Myh6 and Tnnt2 (*p<0.05 vs. d0 undifferentiated; n = 3).