GRA7-I and -III showed anti-mycobacterial activity against MTB challenge <i>in vivo</i>.

<p>Schematic of the TB model treated with rGRA7 or vehicle (<b>A</b>, upper). (<b>A</b>) Bacterial loads in lung, liver, and spleen in PKCα^+/+ and PKCα^-/- mice. <i>n</i> = 10. (<b>B</b>) Histopathology scores were obtained from H&E stained lung sections (left), as described in <b>Methods</b>. Number of granulomas observed in 10 different lung sections per mouse (right). Scale bar, 500 μm. (<b>C</b>) Bacterial loads in lung, liver, and spleen in PLD1^+/+ and PLD1^-/- mice. <i>n</i> = 10. (<b>D</b>) Number of granulomas observed in 10 different lung sections per mouse. Black bars represent the median values. Student’s <i>t</i>-test and Grubbs’ outlier test were used for statistical analysis. The data are representative of two independent experiments with similar results. Significant differences (*<i>P</i> < 0.05; **<i>P</i> < 0.01; ***<i>P</i> < 0.001) compared with PKCα^+/+ and PLD1^+/+.</p>