ppat.1008863.g003.tif (281.39 kB)
Download file

Functional expression of Phh-GluCl and Phh-RDL channels in X. laevis oocytes.

Download (281.39 kB)
figure
posted on 18.02.2021, 19:39 by Nicolas Lamassiaude, Berthine Toubate, Cédric Neveu, Pierre Charnet, Catherine Dupuy, Françoise Debierre-Grockiego, Isabelle Dimier-Poisson, Claude L. Charvet

A. Current traces recorded on oocytes injected with Phh-GluCl cRNA in response to the application of 1 to 1000 μM of glutamate. Oocytes were clamped at -80 mV. Application times are indicated by the bar. B. Glutamate concentration-response curve for Phh-GluCl (mean +/- SEM, n = 5 oocytes). Data were normalized to the maximal effect of glutamate. C. Reverse potential-ion concentration relationships of oocytes expressing Phh-GluCl obtained from voltage-ramps in presence of glutamate and using increasing concentrations of either Cl- or Na+ to analyze the selectivity of this channel. D. Current traces recorded on oocytes injected with Phh-RDL cRNA in response to the application of 1 to 100 μM of GABA. Oocytes were clamped at -60 mV. Application times are indicated by the bars. E. GABA concentration response curve for Phh-RDL (mean +/- SEM, n = 6 oocytes). Data were normalized to the maximal effect of GABA. F. Reverse potential-ion concentration relationships of oocytes expressing Phh-RDL obtained from voltage-ramps in presence of GABA and using increasing concentrations of either Cl- or Na+ to analyze the selectivity of this channel.

History